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PRODUCT SPECIFICATIONS

Produced in closed photobioreactors, in Europe (Portugal)

Gently spray dried and stored at room temperature

Does not contain GMOs or Allergens. No animal ingredients have been used. The product has not been irradiated.

 

ORGANOLEPTIC SPECIFICATIONS

Odor

Characteristic smell

Appearance

Chlorophyll-green fine and soft powder, not aggregated

T aste

Like seaweed

Size of Grains | Mesh

≤ 90 μm

MACRONUTRIENTS (g / 100g)

Carbohydrates

2,0

Dietary fiber

19,3

Fat

9,93

Protein

55,0

Ash

8,25

Moisture

5,5

Energy Value

356

PIGMENTS / CAROTENOIDS (mg / 100g)

Lutein

261

Zeaxanthin

7,8

Chlorophyll

2082

Alpha-carotene

9,5

Beta-carotene

43,3

FATTY ACIDS (g / 100g)

 

Saturated fatty acids

 2,18

Mono-unsaturated fatty acids

2,95

Polyunsaturated fatty acids

4.8

Omega-3-fatty acids

2,15

Omega-6-fatty acids

1,95

Omega-9-fatty acids

1,69

Palmitic acid (C 16:0)

1,70

Palmitoleic acid (C 16:1)

0,74

Stearic acid (C 18:0)

0,18

Oleic acid (C 18:1w9c)

1,65

Linoleic acid (C 18:2w6cc)

1,89

Alpha-linolenic acid (C 18:3w3c)

2,13

MINERALS / TRACE ELEMENTS (mg / 100g)

Sodium

417

Phosphorous

756

Potassium

1790

Calcium

856

Selenium

<0,5

Iron

115

Magnesium

218

Zinc

6,3

Copper

2,36

Iodine

0,019

VITAMINS (/100G)

 

Vitamin A | Retinol

172 μg

Vitamin B1 | Thiamin

<0,1 mg

Vitamin B2 | Riboflavin

0,71 mg

Vitamin B3 | Niacin

16,7 mg

Vitamin B9 | Folic Acid

<5 mg

Vitamin B6 | Pyridoxal phosphate

<10 mg

Vitamin B5 | Pantothenic Acid

5,8 mg

Vitamin B12 | Cianocobalamin

0,88 mg

Vitamin B7 | Biotin

47,8 μg

Vitamin E | Tocopherol

6,8 mg

Vitamin C | Ascorbic Acid

22 mg

AMINOACIDS (g / 100g)

 

Glutamic Acid

5.3

Methionine

0,84

Proline

2,16

Lysine

3,02

Tyrosine

1,53

Phenylalanine

2,21

Serine

1,90

Hystidine

0,75

Glycine

2,84

Threonine

2,35

Arginine

2,78

Alanine

3.89

Valine

2,86

Aspartic acid

4,43

Tryptophan

0,92

Isoleucine

2,06

Leucine

4,13

Cistine

0,12

MICROBIOLOGICAL SPECIFICATION 

Aerobic Plate total counts 30oC

2,6 x 10^3

Staphylococcus aureus counts

< 1,0 x 10^1

Listeria monocytogenes

Not detected / 25 g

E. coli

< 1,0 x 10^1

Salmonella

Not detected / 25 g

 Pseudomonas spp.

< 1,0 x 10^1

Yeasts

2,1 x 10^2

Moulds

< 4,0 x 10^1

Vibrio sp.

Not detected / 25 g

HEAVY METALS

 

Lead

0,29 mg/kg

Cadmium

<0,02 mg/kg

Mercury

< 0,1 mg/kg

Arsenic

< 0,2 mg/kg

Tin

< 1,0 mg/kg

MICROCYSTINES

Microcystin LR

< 0,003 μg/g

MYCOTOXINES

 

Aflatoxin B1; B2; G1; G2

< 0,3 μg/kg

Sum of Aflatoxins

< 1,2 μg/kg

Deoxynivalenol

< 50,00 μg/kg

Patulin

< 5,00 μg/kg

Zearalenone

< 15,00 μg/kg

DIOXINES AND FURANS

PCDD + PCDF

0 – 0,06 pg/g

Sum of Polycyclic aromatic hydrocarbon

0,58 – 0,87 μg/kg

Polychlorinated Biphenyls

0 – 0,29 pg/g

PESTICIDES

Over 200 were tested

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STABILITY AND STORAGE

Sensitive to sunlight, high temperatures and humidity.
Store in the original package in a dry and fresh place. Shelf life of at least 24 months at a temperature below 25°C.
After opened, consume within 6 months

PACKAGING

MATERIAL

FORMAT

CHARACTERISTICS

Primary packaging

Aluminum Foil Bag

250 g

Stand-up Pouch sealed with oxygen absorbers

5 kg

Stand-up Pouch sealed with oxygen absorbers

10 kg

Quadseal bags with oxygen absorbers

Secondary Packaging

Cardboard Box

250 g

16 bags sealed in a 4 kg Cardboard Box

5 kg

  

1 bag sealed in a 5 kg Cardboard Box

   

APPLICATIONS / RECOMMENDATIONS FOR USAGE

Premix formulations for animal feed
Food & Beverage Ingredient or Supplement for human nutrition Cosmetic formulations

MANUFACTURING PROCESS

The Process consists on the scale-up of microalgae. Cells are collected from Nature (endemic species) or may be purchased to certified Laboratories. Each step requires inoculation of the microalgae in a bigger water volume, until the last stage: photo bioreactors (PBR) inoculation.

PBR’s consist of a deposit connected via collectors to a zone of transparent acrylic tubes where the microalga does photosynthesis. This allows the deposit to function as the mixing zone of the culture that circulates in the PBR’s tubes, as a reception point of nutrients, treated water and recirculated water, as a carbonation point (injection of pure CO2) and degassing (gaseous effluent liberation after O2 production and uptake of carbon dioxide, nitrogen and sulfate), as a thermoregulation zone through a spiral heat exchanger and a zone of online monitoring of various parameters like temperature, pH, conductivity and concentration level. Microalgae grow in a medium containing a mixture of nutrients.

When the concentration of cells reaches a peek in the PBRs, the culture is collected and the water is separated from the biomass through a cross-flow ultrafiltration process. The harvest is done in a continuous mode.

The water used in the unit comes from a water hole, passes through a pre-filtration system and is stored in two tanks with the total volume of 500m3. From this point, the water is directed to the treatment system that involves microfiltration, chlorination, filtration by activated carbon and another microfiltration stage.

After treatment, the water may be forwarded to the tanks of water that are used for cleaning the PBR’s inside, to the recirculated water tanks, to the effluent treatments tanks, for filling the production systems (PBR’s), and part for a new filtration stage at 0,2 μm. This water that passes through the new filtration stage feeds the circuit of the inoculation system, the nutrient preparation system and the laboratory where the inoculum is prepared.

The microalgae culture is harvested from the production units (PBRs), and is sent through gravity to a small tank located at a lower level. Hence the culture is pumped to three storage tanks and then concentrated in a tangential filtration system of polymer membranes. The water removed in this process, free of biomass, is recirculated and fed back to PBR’s. Subsequently, the concentrated biomass is sent to the drying system – first, it is collected in a tank to be fed to a spray-dryer and in the dryer, it is atomized into fine droplets to a chamber where circulates hot air, which enters the dryer at about 250oC, leaving wet at about 90oC. After the dryer, the air passes through a cyclone to remove particles. After the drying step, the biomass powder is collected, packaged and stored.